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. 2021 Nov 1;12(11):1040. doi: 10.1038/s41419-021-04335-x

Fig. 6. CaMKK2 expression regulates COPI coatomer expression and its re-expression can rescue the growth defect and Golgi expansion observed in CAMKK2 knockdown cells.

Fig. 6

A LNCaP cells stably expressing control or CAMKK2-targeting shRNA were transfected with EGFP or CaMKK2-EGFP and cultured for 96 h. The growth of the CAMKK2 knockdown cells were normalised to control and the fold change to the seeding density was plotted. Mean ± SD; N = 5 with three technical repeats; **P = 0.0017. B Control and CAMKK2 knockdown LNCaP cells were transiently transfected with control EGFP or CaMKK2-EGFP for 48 h, cells were harvested and cell extracts were subjected to western blots for COPI coat proteins. C Confocal images of control and CAMKK2 knockdown LNCaP cells transiently transfected with EGFP or CaMKK2-EGFP for 48 h, stained with TGN46 antibody and DAPI (nucleus). Scale bar = 25 µm (for all images). D Quantification of Golgi (TGN46-positive) area normalised to cell area of samples shown in C. The EGFP-control transfected CAMKK2 knockdown cells had a significantly (P < 0.0001) larger Golgi area compared to the control. Mean ± SEM; ****P < 0.0001, **P = 0.0017. Two independent experiments with a minimum of 20 cells per group were analysed.