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. 2021 Nov 1;11:21380. doi: 10.1038/s41598-021-00973-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution and duration of circulating gametocyte clones. At each time point analyzed [Day (D)] the different gametocyte clones (n = 11) detected in the subpopulation (n = 149) of infected samples tested are shown. Clonal frequency and distribution differed between time points. The highest number of diverse clones were observed on days 56 and 70. Clones below 300 bp (180–280) were least represented and observed only after day 28.