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. 2021 Nov 1;11:21358. doi: 10.1038/s41598-021-00765-x

Figure 2.

Figure 2

Engineered exosomes were immunogenic for activating RSV-specific CD8 T cells ex vivo. (A) Schematic illustration of the establishment of a DC-CD8 T cell co-culture model. C57BL/6J mice were infected intranasally with RSV strain A2 to generate RSV-specific CD8+ T cells. Magnetic-activated cell sorting (MACS) was used to sort CD8+ T cells from RSV-infected mice and CD11c + DCs from non-infected mice. CD8+ T cells and CD11c + DCs (5:3 ratio) were incubated for 72 h with escalating concentrations (5 μL, 10 μL, 25 μL) of exosomes engineered with M187–195 or NS161–75 peptides with or without LPS (100 ng/mL). (B, C) Cell culture supernatants were collected on day 3 of stimulation with M187–195 or NS161–75 engineered exosomes. The samples were diluted 1:4 and analyzed by commercial ELISA for IFN-γ. Data represent means ± SEM. ****p < 0.0001 as determined by one-way ANOVA and Dunnett's multiple comparisons test. *p < 0.05 as determined by two-way ANOVA and Šídák's multiple comparisons test.