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. 2021 Nov 1;11:21358. doi: 10.1038/s41598-021-00765-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The production of IFN-γ by M_187–195 or NS1_61–75-specific T cells in the lungs. Groups of vaccinated mice were sacrificed either 3 or 6 days post-infection (dpi). Their lungs were stained with antibodies and analyzed by flow cytometry. (A) Representative flow cytometry plots showing IFN-γ producing lungs from a group of exosomes from LPS stimulated JAWS with Quil A at 6 dpi of RSV after stimulation with M_187–195 or NS1_61–75 peptides. (B) Flow cytometric analysis of IFN-γ production by lungs in the CD45+ CD8+ T cell population after stimulation with the indicated conditions. Data are presented as means ± SEM. **p < 0.01 as determined by two-way ANOVA and Tukey's multiple comparisons test.