FIGURE 2.

Effect of serum containing Xihuang pills (XS) on stem cell colony formation of TNBC cells. (A) MTT analysis indicated that the proliferation of breast cell was not inhibited by XS treatment in 48 h. (B) XS increased mammosphere colony formation in TNBC cell lines (4T1 and HCC1806) compared to control serum (CONT). (C) Representative photomicrographs of mammospheres formed by 4T1 and HCC1806 cells treated with serum containing Xihuang pills and control serum (CONT) are shown. Scale bar 50 µM. (D,E) 4T1 and HCC1806 cells were treated with 0, 0.1, 1, 4, 16, 64,256, or 1024 nM PTX for 48 h. MTT assay was used to detect the viability of cells (XS compared to CONT). *p < 0.05, **p < 0.01, ***p < 0.001 vs the control group.