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. 2021 Nov 1;4:1241. doi: 10.1038/s42003-021-02772-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c PC9 and LK2 were treated with AZD4785 for 24 h. For the pulse-chase (p/c) experiment, cells were treated with 10 µM AZD4785 for 6 h, washed and incubated for 24 h in the absence of AZD4785 (AZD4785 p/c). EVs were isolated from conditioned cell media by differential ultracentrifugation and a 100,000×g pellets were analysed by NTA. Representative NTA images. (N = 6). b Size distribution of EVs secreted by PC9 and LK2 cells. EVs were collected and analysed as in a. Error bars, standard deviation. ANOVA ****p < 0.0001. ***p < 0.001. ns non-significant. N = 3–6. c Quantification of EV secretion by PC9 and LK2 cells. The total number of EVs was measured by NTA as in a and divided by the total number of cells. Error bars, standard deviation. ANOVA. ****p < 0.0001, ns non-significant. N = 3–6. d Detection of exosomal markers, CD63 and Alix, in EVs isolated from PC9 and LK2 cells after 24 h treatment. Equal aliquots of EVs isolated as in a were analysed by western blotting and probed for CD63 and Alix. Representative image from N = 2. e Vein diagram for the EV’s protein mass spectrometry analysis revealed differentially secreted protein in PC9 and LK2 EVs.