Fig. 6. S100B inhibits toxic seeding mediated by tau oligomers.

a, b Representative immunofluorescence images of tau RD P301S FRET biosensor cells exposed to tau oligomers (0.1 µM and 0.5 µM) alone (a) or to tau oligomers preincubated with S100B (5 µM) (b) for 24 h in the presence of lipofectamine. In all, 5 µM S100B data are shown as representative data of the effect of S100B alone in tau biosensor cells, as even higher concentrations did not cause seeding. Cell nuclei stained with DAPI (blue). Objective used: 40X; scale bar 50 μm. c Statistical analysis with data presented as mean and standard error of mean from n ≥ 3 independent experimental replicates using one-way analysis of variance (ANOVA) with Tukey’s multiple-comparison test. Specifically, for assays with vehicle and 5 µM S100B n = 3, for assays with 0.1 µM TauO n = 5, for assays with 0.5 µM TauO n = 4, and for assays with 5 µM S100B + 0.1 µM TauO and 5 µM S100B + 0.5 µM TauO n = 6. Statistical significance at p < 0.05 was considered, ****p < 0.0001 and ***p = 0.0009.