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. 2021 Nov 1;7:329. doi: 10.1038/s41420-021-00724-5

Fig. 2. The overexpression of FTO promoted bladder cancer cell proliferation and invasion in vitro and in vivo.

Fig. 2

A FTO was detected by western blotting after FTO overexpression in T24 and UM-UC-3 cells. B The m6A levels were measured in FTO-overexpressing T24 and UM-UC-3 cells. C FTO overexpression promoted cell proliferation determined by CCK-8 assays. D Transwell migration and invasion assays after FTO overexpression at a 100× magnification. Scale bars represent 100 μm. E Cell-cycle analysis was performed in FTO-overexpressing T24 and UM-UC-3 cells using flow cytometry. F Image of tumours collected from nude mice. The tumour growth curve and tumour weight were measured in the FTO-overexpression group and the control group. The results are presented as mean ± standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001.