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. 2021 Nov 1;12:6277. doi: 10.1038/s41467-021-26506-2

Fig. 1. Schematic of the native S protein of SARS-CoV-2 and S gene constructs.

Fig. 1

a The native S protein is 1273 amino acids (aa) in length. The protein contains 2 subunits, S1 and S2, generated by cleavage at the furin cleavage site (F). S1 contains the signal peptide (SP), N-terminal domain (NTD) and receptor-binding domain (RBD). S2 contains the fusion peptide (FP), heptad repeats 1 (HR1) and 2 (HR2), transmembrane domain (TM), and cytoplasmic tail (CT). The 2P indicates the two mutated prolines, K986P and V987P. The green letters indicate the endoplasmic reticulum retrieval signal (ERRS) motif, KxHxx, in the CT. dER indicates constructs carrying a deletion of the 11 C-terminal amino acids from the CT. b The native S gene of SARS-CoV-2 with notable domains indicated in color boxes relative to the S gene constructs cloned into the MV vector. 2P and dER modifications indicated by the red boxes. All S constructs were cloned into either the second (ATU2) or third (ATU3) additional transcription units of pTM-MVSchwarz (MV Schwarz), the MV vector plasmid. The MV genome comprises the nucleoprotein (N), phosphoprotein (P), V and C accessory proteins, matrix (M), fusion (F), hemagglutinin (H) and polymerase (L) genes. Plasmid elements include the T7 RNA polymerase promoter (T7), hammerhead ribozyme (hh), hepatitis delta virus ribozyme (∂), and T7 RNA polymerase terminator (T7t).