Fig. 4. Induction of S-specific cellular responses by rMV vaccination.

a IFNAR^−/− mice were immunized by intraperitoneal injection with 1 × 10⁵ TCID₅₀ of MV-ATU2-SF-2P-dER, n = 11; MV-ATU3-SF-2P-dER, n = 8; MV-ATU2-S2-2P-dER, n = 9; MV-ATU3-S2-2P-dER, n = 9 and Empty MV, n = 3. Seven days after immunization, ELISPOT for IFNγ was performed on freshly extracted splenocytes. The data are shown as IFNγ-secreting cells or spot-forming cells (SFC) per 1 × 10⁶ splenocytes detected after stimulating with b MV Schwarz or c SARS-CoV-2 S peptide pools specific to CD8⁺ or CD4⁺ T cells. d Ratio of IFNγ-secreting cells stimulated by CD4⁺ or CD8⁺ peptides to those stimulated by MV Schwarz. Each data point represents an individual mouse. Data are represented as mean with line and error bars indicating ±SD. Significant differences between the groups were determined by two-tailed the Mann–Whitney test.