. 2021 Oct 19;9:741444. doi: 10.3389/fchem.2021.741444

TABLE 2.

Antioxidant activity of donepezil hybrids: effects of donepezil hybrids against ROS production induced by H₂O₂ in SH-SY5Y cell cultures; ORAC and CUPRAC assay.

Entry	Compound	ROS measurement ^a						ORAC ^b	CUPRAC ^c
Entry	Compound	CTRL	H₂O₂ (100 μM)	250 nM	500 nM	1 μM	10 μM	ORAC ^b	CUPRAC ^c
1	HT1	1	6.31	6.18	5.25	5.47	5.49	21.2 ± 1.6	3.36
2	HT1a	1	6.31	2.99	2.63	2.51	2.66	n.t. ^d	n.t. ^d
3	HT2	1	6.31	2.21	1.97	1.12	1.38	7.6 ± 0.2	2.77
4	HT3	1	6.31	3.68	2.91	2.18	2.76	45.2 ± 3.6	1.80
5	HT3a	1	6.31	2.27	1.94	1.58	2.13	n.t. ^d	n.t. ^d
6	HT4	1	6.31	3.9	3.2	3.64	3.67	23.0 ± 2.0	0.99
7	HT4a	1	6.31	2.34	2.77	2.94	3.01	n.t. ^d	n.t. ^d
8	HT	n.t.	n.t.	n.t.	n.t.	n.t.	n.t.	38.8 ± 4.4	2.82
9	Trolox	n.t	n.t.	n.t.	n.t.	n.t.	n.t.	—	1

The fluorescence values (Fitch) obtained from cytometric analysis were processed and the related quantification was conducted by setting the value of the control to 1 and comparing all other values.

Data expressed as means ± SEM of three independent observations (µmol trolox l⁻¹).

Data are expressed as (mmol of trolox)/(mmol of tested compound).

n.t. = not tested.