Subject	Neuroscience: Neurophysiology
Specific subject area	Cellular calcium dynamics. Substantia nigra dopaminergic neurons.
Type of data	Raw data: output of the CCD chip used to measure calcium-induced changes in fura-2 absorption.
How data were acquired	Fluorescence measurements were performed with fura-2 using an Imago/SensiCam CCD camera with a 640 × 480 chip (Till Photonics, Gräfelfing, Germany) and a Polychromator IV (Till Photonics). Both were mounted on an upright microscope. The Substantia nigra dopaminergic neurons were recorded in brain slices of adult mice.
Data format	HDF5
Parameters for data collection	Regularly timed excitations at three different wavelengths allowed the calcium dynamics to be monitored. The data are collections of time series recorded at 340, 360, and 380 nm excitation.
Description of data collection	Two different protocols were used for loading the calcium dye (fura-2) into the neurons: the ‘conventional’ whole-cell and the β-escin based perforated patch configuration.
Data source location	University of Cologne, Institute for Zoology Cologne, NRW Germany
Data accessibility	Repository name: Zenodo Data identification number: 4326607 Direct URL to data: https://zenodo.org/record/4326607
Related research article	S. Hess, C. Pouzat, L. Paeger, A. Pippow, P. Kloppenburg, Analysis of neuronal Ca2+ handling properties by combining perforated patch clamp recordings and the added buffer approach, Cell Calcium, 2021 May 10;97:102411. https://doi.org/10.1016/j.ceca.2021.102411 [3]