Figure 5.

S1R topology does not change in the presence or absence of S1R agonists.A, S1R−/− HEK293 cells expressing WT S1R or the N-glycosylation site constructs were treated with 100 nM Pre-084 for 16 h or left untreated. Cell lysates were immunoblotted with anti-S1R. No change is observed in the glycosylation patterns. B, similar to (A) but using 3 μM pridopidine instead of Pre-084.