Figure 6.

S1R is ER-retained in the presence or absence of ER stress or of S1R agonists.A, lysates of S1R−/− HEK293 cells expressing WT S1R or the N-glycosylation site constructs were treated with or without endo H and immunoblotted with anti-S1R. The indicated samples were treated with 5 μg/ml BFA for 16 h before lysis, causing partial endo H resistance (two right lanes). B, similar to (A), but with cells treated with 100 nM Pre-084 for 16 h prior to lysis or left untreated. No change is caused by Pre-084 in the glycosylation patterns. C, similar to (B), but with cells treated with 3 μM pridopidine or left untreated. D, similar to (B), but with cells treated with 2 μg/ml TG for 16 h or left untreated.