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. 2021 Oct 6;297(5):101281. doi: 10.1016/j.jbc.2021.101281

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Role of TMEM2 in dynamic HA degradation in the lymphatic system. A, time course analysis of lymphatic HA degradation in wild-type mice. FA-HA (100∼300 kDa) was injected subcutaneously into the left hindlimb paw of adult wild-type mice, and the degradation pattern of FA-HA in the popliteal lymph node at 1 and 6 h after the injection was analyzed by Sephacryl S-300 chromatography. B, degradation patterns of FA-HA analyzed by gel filtration on Sephacryl 300 HR. Upper panel, analysis of hindlimb-injected FA-HA in the popliteal lymph nodes; lower panel, analysis of forelimb-injected FA-HA in the axillary lymph nodes. Elution profiles were normalized to the total fluorescence counts eluted from the column. Tmem2^iKO (red); control (blue).