Table 1.

Reference of constituents of various types of cell media used for this study

Medium type	Basal medium type	Additive 1 /Dilution or concentration	Additive 2 /Dilution or concentration	Notes
RPMI-20 (for replating hiPSC-CMs)	RPMI-1640 with glucose	Fetal bovine serum – 20% (V:V)	Y-27632 (5 μM) – add immediately before use	Store at 4°C for up to 1 month. Y-2 is only necessary when plating cells (not for halting digestion)
RPMI-1640 (for hiPSC-CM culture)	RPMI-1640 with glucose	B-27™ supplement (with or without insulin as specified in the protocol) 1:50 dilution (V:V)		Store at 4°C for up to 1 month.
DMEM with lactate (for hiPSC-CM selection)	DMEM, glucose-free	1M (L+)-lactic acid solution - 1:250 dilution (V:V) – final [] = 4mM		Store at 4°C for up to 1 month.
50× (500 mM) galactose solution	RPMI-1640 without glucose	Galactose = to final concentration of 500 mM: 45.1 g/500mL		See “step-by-step method details” for solution preparation. Store at −20°C for up to 3 months
Na2 EGTA (10mL) - used for myofibril lysis buffer	MQ H2O	0.380 g EGTA powder	0.08 g NaOH powder	pH to 7 with KOH. filter with 0.4 μm filter. Store at −20°C for up to 1 year.
1 M K-prop solution(10mL) - used for myofibril lysis buffer	10 mL 1M propionic acid solution	0.561 KOH powder		Mix KOH into propionic acid and filter with 0.4 μm filter. Store at −20°C for up to 1 year.