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50× fatty acid solution
| Reagent | Final concentration | Amount (for 10 mL) |
|---|---|---|
| RPMI-1640 without glucose | N/A | 10 mL |
| BSA Fraction V (Fatty acid free) | 10% (W:V) | 1 g |
| 250 mM palmitic acid | 2.5 mM (1:100 dilution) | 100 μL |
| 500 mM oleic acid | 5 mM (1:100 dilution) | 100 μL |
See “step-by-step method details” for fatty acid conjugation. Store at −20°C for up to 3 months.
