Table 3.

Maturation medium (for hiPSC-CM maturation)

Reagent	Final concentration	Amount (for 500 mL)
RPMI-1640 without glucose	N/A	470 mL
50× fatty acid solution	1× (100 μM oleic acid, 50 μM palmitic acid)	10 mL
50× galactose solution	1× (10 mM galactose)	10 mL
50× B-27™ supplement with insulin	1×	10 mL

See “step-by-step method details” for solution preparation. Filter w/ 0.22 μm filter before use. Good for 2–3 weeks if stored at 4°C