FIGURE 7.

Obesity‐induced adipose tissue inflammation was reduced in 7E‐treated mice. Male C57BL/6 mice were randomly assigned to the LFD (10% kcal derived from fat) or the HFD (60% kcal derived from fat) group for 16 weeks. The experiments began 1 week after HFD and the mice were divided into three groups (n = 10 mice per group): HFD mice without treatment, and HFD mice treated with 10 mg mIgG/kg/3 d or 10 mg 7E/kg/3 d. The number of ATMs (a), M1 macrophages (b) and M2 macrophages (c) in adipose tissue isolated from mIgG‐treated and 7E‐treated HFD mice were calculated using FACS analysis. (d) RT‐qPCR analysis of MIF, TNF‐α and MCP‐1 mRNA expression in adipose tissue isolated from mIgG‐treated and 7E‐treated HFD mice. (e) IHC staining of IL‐20 in adipose tissue section from mIgG‐treated and 7E‐treated HFD mice. Data are expressed as mean ±SEM and are representative of three independent experiments. *p < 0·05 compared with mIgG‐treated HFD mice