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. 2021 Oct 11;2(10):100408. doi: 10.1016/j.xcrm.2021.100408
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological samples

Human plasma Clinical Trial NCT-03096535
Human abdominal subcutaneous fat biopsies Clinical Trial NCT-03096535

Chemicals, peptides, and recombinant proteins

TaqMan Universal PCR Master Mix Thermo Fisher Scientific Cat# 4305719
PowerUp SYBR Green Master Mix Thermo Fisher Scientific Cat# A25777
Trizol Thermo Fisher Scientific Cat# 15596026
18-F-FDG- radiotracer Department of Nuclear Medicine, Rigshospitalet N/A

Critical commercial assays

R-plex Human adiponectin MSD assays Meso Scale Discovery, Rockville, Maryland F21YT
Human Cortisol Parameter Assay Kit RND Systems KGE008B
U-plex human leptin MSD assays Meso Scale Discovery, Rockville, Maryland K1515ZK
Human V-PLEX IL-6 MESO QuickPlex SQ 120 Meso Scale Discovery, Rockville, Maryland K0081460
High-Capacity cDNA Reverse Transcription kit Thermo Fisher Scientific Cat# 4374966

Oligonucleotides

CKMT2 Thermo Fischer Scientific/Applied Biosystems Hs00176502_m1
PLIN1 Thermo Fischer Scientific/Applied Biosystems Hs00160173_m1
PPARGC1A: forward 5′-caagccaaaccaacaactttatctct-3′; reverse 5′-cacacttaaggtgcgttcaatagtc-3′ Biosearch Tecnologies https://eu.biosearchtech.com/
TBX1: forward 5′- acgacaacggcc-3′; reverse 5′- cctcggcatatt Biosearch Tecnologies https://eu.biosearchtech.com/
TMEM26: forward 5′- atggagggactg-3′; reverse 5′- cttcacctcggt-3′ Biosearch Tecnologies https://eu.biosearchtech.com/
LIPE: forward 5′- ctgctcctccgagacttcc-3′; reverse 5′- gacttgcgcccacttaactc Biosearch Tecnologies https://eu.biosearchtech.com/
ELOVL6: forward 5′- caaagcacccgaactaggag-3′; reverse 5′- tggtgataccagtgcaggaa-3′ Biosearch Tecnologies https://eu.biosearchtech.com/

Software and algorithms

ResearchIR 4.2 software FLIR Software https://www.flir.com/
SAS 9.1 SAS Institute https://www.sas.com/en_us/home.html
Electromyography (EMG) MATLAB https://www.mathworks.com/
Prism v9.0 GraphPad http://www.graphpad.com/

Other

Dual X-ray absorptiometry (DXA) Lunar Prodigy Advance; GE Healthcare, Madison, WI, USA https://www.gehealthcare.com/products/bone-health-and-metabolic-health
Indirect calorimetric system Quark B2, Cosmed, Italy https://www.cosmed.com/en/products/indirect-calorimetry/quark-rmr
FLIR camera A655sc FLIR Systems AB, Presicion Nordic, https://www.flir.com/
The Blanketrol III Cincinnati Sub-Zero Blanketrol III Hyper-Hypothermia System Model: 233 https://www.cszindustrial.com/
Thermochron iButtons® Maxim DS1921H-F50
e-Celsius® Performance capsule Ingestible pill https://www.bodycap.us/