FIG. 6.

Evi9 colocalizes with BCL6 but not with PML or Sp100. GFP-tagged Evi9a was cotransfected along with expression constructs of BCL6 (A to C), PML (D to F), or Sp100 (G to I) into HeLa cells. Evi9 localization was detected as GFP signals in panels A, D, and G. Polyclonal antisera against BCL6 (B), PML (E), or Sp100 (H) were also used, and the signals were detected using rhodamine-conjugated anti-rabbit immunoglobulin G. Colocalization was evaluated by merging the different images (C, F, and I). Colocalization of Evi9a and BCL6 was also confirmed by using nontagged Evi9a and Myc-tagged BCL6 constructs with anti-Evi9 Ab1 antisera (J) and anti-Myc epitope antibody (K). Colocalization was noted by merging the images (L).