Figure 3.

IFN-γ producing, hyperproliferative gastric epithelial cells using MIBI-TOF

(A) Color overlay of IFN-γ⁺ cells (magenta), pan-CK⁺ epithelial cells (yellow), and major histocompatibility complex (MHC) class II cells (light blue) in a representative region of gastric biopsy, accompanied by CD45 and CD3 expression in the same region.

(B) Localization of IFN-γ protein in 2 different representative regions of gastric biopsies (left). Random areas with IFN-γ⁺ cells were selected and expression of pan-CK, IFN-γ, and CD45 is displayed (right).

(C) Color overlay of CD45⁺ pan-leukocyte marker (magenta), Ki-67⁺ proliferating cells (yellow), and pan-CK⁺ epithelial cells (light blue) in a representative region of gastric biopsy, accompanied by MHC class II and IFN-γ expression in the same region. Light blue arrows signify the presence (shorter arrow) or absence (longer arrow) of IFN-γ and Ki-67 co-expression in epithelial cells.

(D) Stacked bars depicting the relative abundance of IFN-γ ⁺ cells across all ROIs and cell types.

(E) In situ hybridization in a serial gastric tissue section. Four representative regions are displayed.

HH3, histone H3; Pan-CK, pan-cytokeratin.

See also Figure S2.