Table 2.
Susceptibility of imipenem-non-susceptible bacteria with different genotypes and phenotypes to β-lactams with and without novel BLI-BLEs
Species with different genotypes and phenotypes | N | Susceptible (%) |
|||||||
---|---|---|---|---|---|---|---|---|---|
FEPa | FPZ | CAZ | CZA | IPM | IMR | MEM | MEV | ||
E. coli and K. pneumoniae | 180 | 23.3 | 99.4 | 3.3 | 88.9 | 0 | 75.6 | 30 | 82.8 |
genotype | |||||||||
with carbapenemase gene | 92 | 5.4 | 100 | 2.2 | 82.6 | 0 | 59.8 | 7.6 | 70.7 |
with class B carbapenemase gene | 13 | 0 | 100 | 0 | 0 | 0 | 0 | 7.7 | 53.8 |
with blaKPC-like only | 58 | 0 | 100 | 0 | 96.6 | 0 | 84.5 | 0 | 75.9 |
with blaOXA-48-like only | 20 | 25 | 100 | 10 | 95 | 0 | 30 | 30 | 70 |
without carbapenemase gene | 88 | 42 | 98.9 | 4.5 | 95.5 | 0 | 92 | 53.4 | 95.5 |
with ESBL genea only | 1 | 0 | 100 | 100 | 100 | 0 | 100 | 100 | 100 |
with AmpC gene only | 41 | 85.4 | 97.6 | 2.4 | 95.1 | 0 | 95.1 | 63.4 | 95.1 |
with ESBL and AmpC geneb | 43 | 0 | 100 | 0 | 95.3 | 0 | 90.7 | 41.9 | 95.3 |
phenotypec | |||||||||
class B carbapenemase producerd | 11 | 0 | 100 | 0 | 0 | 0 | 0 | 9.1 | 45.5 |
non-MBL producer | 82 | 7.3 | 100 | 2.4 | 93.9 | 0 | 68.3 | 8.5 | 74.4 |
non-carbapenemase producer | 87 | 41.4 | 98.9 | 4.6 | 95.4 | 0 | 92 | 52.9 | 95.4 |
ESBL producer | 22 | 4.5 | 100 | 4.5 | 100 | 0 | 100 | 72.7 | 100 |
A. baumannii | 136 | 1.5 | 8.1 | 2.2 | 1.5 | 0 | 0.7 | 0 | 0 |
genotype | |||||||||
with class D carbapenemase gene | 135 | 1.5 | 8.1 | 2.2 | 1.5 | 0 | 0 | 0 | 0 |
blaOXA-23-like | 117 | 0 | 6.8 | 1.7 | 0.9 | 0 | 0 | 0 | 0 |
blaOXA-24-like | 22 | 4.5 | 13.6 | 0 | 4.5 | 0 | 0 | 0 | 0 |
P. aeruginosa | 81 | 45.7 | 91.4 | 43.2 | 81.5 | 0 | 85.2 | 28.4 | 42.0 |
genotype | |||||||||
with class B carbapenemase gene | 4 | 0 | 75 | 0 | 0 | 0 | 0 | 0 | 0 |
without carbapenemase gene | 77 | 48.1 | 92.2 | 45.5 | 85.7 | 0 | 89.6 | 29.9 | 44.2 |
FEP, cefepime; FPZ, cefepime/zidebactam; CAZ, ceftazidime; CZA, ceftazidime/avibactam; IPM; imipenem; IMR, imipenem/relebactam; MEM, meropenem; MEV, meropenem/vaborbactam.
SDD and susceptible breakpoints (≤8 mg/L) for cefepime were used here.
Only prevalent ESBL genes were tested.
Isolates with positive mCIM and eCIM were defined as class B carbapenemase (MBL) producers; those with positive mCIM but negative eCIM were non-MBL carbapenemase producers; those with negative mCIM were non-carbapenemase producers.
Seven of the 18 isolates positive for both eCIM and mCIM were only positive for blaKPC-like by PCR, which was further confirmed by WGS. Therefore, these seven isolates were categorized as non-MBL carbapenemase producers.