Figure 5.

Pathway classification of proteins increased in level following ZNF648 knockdown. Tandem Mass Tag labeling and nanoscale liquid chromatography coupled to tandem mass spectrometry (nano-LC MS/MS) was performed on whole cell lysates from erythroid cells transduced with ZNF648 or scrambled (Scr) (control) short hairpin RNA (shRNA) at day 8 in culture. Proteins increased in level following ZNF648 knockdown were entered into Reactome. Dark red indicates proteins apportioned to the pathway shown on the left. Top 20 pathways shown with minimum probability of over representation in the dataset, corrected for false discovery rate, of P<2.11x10-5.