FIG 4.

SepA is essential to enhance neutrophil migration and Shigella invasion in the presence of AAT. The significant increases in PMN migration (A) and bacterial invasion (B) stimulated by the presence of AAT are dependent on the expression of SepA, with both effects getting abrogated when cells were infected with a sepA mutant strain (ΔsepA) and restored when the mutant was complemented with a plasmid to express sepA (pZK15). Asterisks denote P values of <0.05 as determined by a 2-tailed nonparametric Mann-Whitney U test. Data are means ± SE from four replicates, and results are representative of experiments with PMNs from different donors. This image was created with BioRender.