TABLE 1.
SepA targets identified by tandem mass spectrometry
| Spot | Protein name (entry name, UniProt) | Function (reference) |
|---|---|---|
| 12 | 2,4-Dienoyl-coenzyme A reductase, mitochondrial (DECR_HUMAN) | Mitochondrial enzyme required for the β-oxidation of unsaturated fatty acids (86) |
| 23 | Gamma-actin (ACTG_HUMAN) | Globular multifunctional protein that constitutes the monomeric unit of cytoskeletal microfilaments (87) |
| 1, 2 | Alpha actinin-4 (ACTN4_HUMAN) | Cytoskeletal protein that participates in the organization of cell cytoskeleton and is adjacent to adherent junctions (88) |
| 5, 6, 24 | Alpha-1 antitrypsin (A1AT_HUMAN) | Serin protease inhibitor important for the inhibition of neutrophil elastase at inflammatory sites to abate incidental destruction of surrounding tissue and to facilitate tissue repair (49) |
| 8, 9 | Annexin A2 (ANXA2_HUMAN) | Multifunctional Ca2+ and phospholipid-binding protein involved in the activation of plasmin, the regulation of membrane dynamic events, and the regulation of inflammatory processes (89) |
| 21 | Anterior gradient protein 2 homolog (AGR2_HUMAN) | Protein disulfide isomerase involved in protein quality control in the endoplasmic reticulum and essential for the production of intestinal mucus (36, 90) |
| 10 | Calreticulin (CALR_HUMAN) | Multifunctional Ca2+-binding protein that operates as a chaperone to assist correct protein folding in the endoplasmic reticulum (91) |
| 20 | Cofilin-1 (COF1_HUMAN) | Actin-binding protein that controls the depolymerization of actin filaments and promotes the opening of tight junctions in the intestinal epithelium (17) |
| 18 | Ferritin light chain (FRIL_HUMAN) | Light subunit of the ferritin protein, important for iron homeostasis (92) |
| 11, 19 | Galectin-4 (LEG4_HUMAN) | Beta-galactoside-binding protein implicated in cell proliferation, apoptosis, differentiation, and intercellular adhesion (93) |
| 17 | Heterogeneous nuclear ribonucleoprotein A2/B1 (ROA2_HUMAN) | RNA-binding protein involved in the transcription, splicing, transport, stability, and translation of a large no. of genes (94) |
| 13 | Heterogeneous nuclear ribonucleoprotein A3 (ROA3_HUMAN) | RNA-binding protein that binds the cis-acting response element A2RE and participates in the cytoplasmic trafficking of RNA (95) |
| 22 | Histidine triad nucleotide-binding protein 1 (HINT1_HUMAN) | Phosphoramidase that regulates the activities of different transcription factors and participates in the regulation of apoptotic pathways (96, 97) |
| 3, 4 | Ras GTPase-activating-like protein IQGAP1 (IQGA1_HUMAN) | Scaffold protein that participates in the modulation of the cytoskeletal architecture, cytokinesis, intracellular signaling, and intercellular interactions (98) |
| 7 | N-Acetyl-d-glucosamine kinase (NAGK_HUMAN) | Sugar kinase necessary to catalyze the conversion of N-acetylglucosamine (GlcNAc) to GlcNAc-6-phosphate, a component that eventually leads to the synthesis of O-/N-glycans and sialic acid (99) |
| 16 | Peroxiredoxin-2 (PRDX2_HUMAN) | Antioxidant enzyme that hydrolyzes hydrogen peroxide molecules to water and protects cells against oxidative damage from reactive oxygen species (ROS) (100) |
| 15 | Protein/nucleic acid deglycase DJ-1 (PARK7_HUMAN) | Redox-sensitive molecular chaperone that inhibits protein aggregation and helps to regulate oxidative stress (101, 102) |
| 14 | Translationally controlled tumor protein (TCTP_HUMAN) | Multifunctional protein involved in cell growth, development, apoptosis, regulation of protein synthesis, DNA repair, immune response, malignant transformation, and tumor reversion (103, 104) |