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. 2021 Jun 25;30(22):2082–2099. doi: 10.1093/hmg/ddab159

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© The Author(s) 2021. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ripk1 localizes to activated macrophage/microglia. (A-I) Combined ISH with RNAscope probe Mm-Ripk1-C1 and fluorescent IHC staining of twi-2J with cell type-specific markers for macrophage/microglia Iba1(A-D), oligodendroglia Olig2 (E), macrophage/microglia Iba1 and astroglia Gfap (F), non-phosphorylated neurofilament protein SMI32 (G), p62 and neuronal marker NeuN (H), and p62 (I). Nuclear stain: DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride. Mutant (mut), wild type (wt), dorsal white column (dwc), grey matter (gm). Scale bars: 50 μm.