FIG. 11.

Time course of the effects of MAb 4D5 treatment on G₁ regulators in MKN7 cells. MKN7 cells were seeded at a density of 3 × 10⁴ cells/cm². After 24 h of incubation, the medium was changed and MAb FRP5 or MAb 4D5 was added to a concentration of 10 μg/ml for the times indicated. Cell extracts were prepared and analyzed by immunoblotting (WB) either directly or after immunoprecipitation (IP) with Cdk2-specific antibodies. Untreated cells (t = 0) and cells treated with MAb FRP5 for 24 h were included as controls.