Abstract
Using a continuous cell line of canine kidney (MDCK-cell line) cells, HCC virus was isolated from 4 of 6 puppies affected in an outbreak of the disease in a dog kennel. The virus was most easily, and rapidly, isolated in primary cultures from the liver samples, but also from other organs such as the spleen and lung, although isolation from these organs could require 1 or 2 passages in addition to the primary culture. Attention is therefore drawn to the necessity of using a sufficient number of passages when organs other than the liver are available. Among the bacteria identified in addition to the virus infection, were Staphylococcus aureus, Bordetella bronchiseptica and β-hemolytic streptococci. Emphasis is made on the significance of isolation and identification of the virus in pure culture, thus enabling its various properties to be studied in detail, and possible new serotypes to be recognized.
Keyword: canine hepatitis virus, canine kidney cell line
Sammendrag
Med bruk av en cellelinje av hundenyreceller (MDCK-celler) ble HCC-virus isolert fra 4 av 6 hvalper under et enzootisk utbrudd i en kennel. Viruset ble isolert i primærkultur fra lever, men også fra andre organer som milt og lunge, selv om isolasjon fra sistnevnte organer ofte krevet 1 til 2 passasjer i tillegg til primærkultur. En fremhever derfor betydningen av et tilstrekkelig antall passasjer i cellekultur når andre organer enn lever anvendes for undersøkelse. Som sekundær bakterieinfeksjon forekom Staphylococcus aureus, Bordetella bronchiseptica og β-hemolytiske streptokokker. En påpeker betydningen av isolasjon av viruset i renkultur for at dets forskjellige egenskaper kan studeres i detalj og eventuelle nye serotyper påvises.
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