Figure 3.

PLOD1-knockout using the CRISPR/Cas9 system suppressed the proliferation of A549 cells. (A) PLOD1 protein expression levels in A549 cells was assessed by western blot analysis using GAPDH as the internal control. (B) Cellular proliferation was assessed by MTT assay at 1, 3, and 5 days of culture. (C and D) Effects of PLOD1 on the proliferation of A549 cells were assessed by cell colony formation capacity. ***P<0.001. PLOD1, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1; WT, wild-type.