Reagent/Resource	Reference or Source	Catalog Number
Experimental Models
143B Osteosarcoma cells (H. sapiens)	American Type Culture Collection	CRL‐8303
Rho0 cells derived from 143B cells	Wittig et al (2010b)
Chemicals, Enzymes and other reagents
DMEM 4.5 g/l Glucose w/o Glutamine and Sodium Pyruvate	Lonza Group	Cat # BE12‐733F
Fetal Bovine Serum (Heat‐Inactivated)	Life Technologies/Gibco	Cat # 10500064
MEM Non‐Essential Amino Acid Solution (100×)	Sigma‐Aldrich	Cat # M7145
L‐Glutamine	Sigma‐Aldrich	Cat # G7513
Sodium Pyruvate	Sigma‐Aldrich	Cat # S8636
Penicillin‐Streptomycin Solution	Sigma‐Aldrich	Cat # P4333
Uridine	Sigma‐Aldrich	Cat # U3003
Trypsin‐EDTA Solution 10×	Sigma‐Aldrich	Cat # 59418C
Coomassie Brilliant Blue G 250	SERVA Electrophoresis	Cat # 17524
6‐Aminohexanoic Acid	Sigma‐Aldrich	Cat # A2504
Digitonin Water Soluble	SERVA Electrophoresis	Cat # 19551
Sequencing Grade Modified Trypsin	Promega	Cat # V5111
Reprosil‐Pur 120 C18‐AQ 3 µM	Dr. Maisch GmbH	Cat # r13.aq.0001
Acetonitrile ULC/MS – CC/SFC	Biosolve	Cat # 012041
Software
MaxQuant 1.4.1.2	https://maxquant.org Cox and Mann (2008)
COPAL 1.0	https://github.com/cmbi/copal van Strien et al (2019)
NOVA 0.8.0	http://www.bioinformatik.uni‐frankfurt.de/tools/nova Giese et al (2015)
GraphPad Prism 8.4.3	http://www.graphpad.com
PyMol Molecular Graphics System Version 2.0	https://pymol.org Schrödinger, LLC
Other
Q‐Exactive Orbitrap MS	Thermo Scientific
EASY‐nLC 1000	Thermo Scientific
100 µM PicoTip Emitter Column	New Objective Inc.	Cat # FS360‐100‐8‐N‐5‐C15
Multiscreen‐BV, 1.2 µm Filter Plates	Millipore	Cat # MABVN1250