Antibody	Source	Identifier
Mouse Monoclonal anti‐Flag M2	Sigma‐Aldrich	F1804
Mouse Monoclonal anti‐flag M2 affinity gel	Sigma‐Aldrich	A2220
Rabbit Polyclonal anti‐Flag	Sigma‐Aldrich	F7425
Rabbit Polyclonal anti‐Histone H3K4me3	Abcam	ab8580
Goat anti‐Histone H3	Abcam	ab12079
Rabbit polyclonal anti‐Histone H4	Active Motif	39269
Rabbit Polyclonal anti‐ATRX	Santa Cruz	sc‐15408
Mouse Monoclonal anti‐BrdU (for FACS) BU20a	Dako	MO744
Purified Mouse Anti‐BrdU (for IF) clone B44	BD Biosciences	347580
Rabbit Polyclonal anti‐Histone H3K9me3	Abcam	ab8898
Rabbit Polyclonal anti‐acetyl Histone H3 (Lys 9/14)	Cell Signaling technology	9677
Mouse Monoclonal anti‐Histone H3K27me3	Abcam	ab6002
Mouse Monoclonal anti‐Histone H4K20me3	Active Motif	39672
Rabbit polyclonal anti‐Histone H4 (acetyl K12)	Abcam	ab46983
Rabbit monoclonal anti‐Histone H4 (acetyl K16)	Abcam	ab109463
Rabbit monoclonal anti‐Histone H4 (acetyl K5)	Abcam	ab51997
Mouse monoclonal anti‐HP1α	Millipore	05‐689
Mouse monoclonal anti‐HP1β	Abcam	ab10478
Mouse Monoclonal anti‐phospho Histone H2A.X (ser139)	Millipore	05‐636
Rabbit Polyclonal anti‐53BP1	Abcam	ab21083
Rabbit monoclonal anti‐HA Tag	Cell Signaling Technologies	3724
Mouse monoclonal anti‐His	Cell Signaling Technologies	2366
Mouse monoclonal anti‐V5	Invitrogen	R962‐25
Mouse monoclonal anti‐HP1γ	Millipore Sigma	MAB3450
Anti‐GST‐HRP conjugate	Millipore Sigma	RPN1236
Rabbit monoclonal anti‐Mad2L2/REV7	Abcam	ab180579
Mouse monoclonal anti‐Chk1 (G‐4)	Santa Cruz	sc‐8408
Rabbit anti‐pChk1 S345 (133D3)	Cell Signaling	2348S
GFP‐Trap	Chemotek	gta‐20
Rabbit anti‐RPA2	Bethyl	A300‐244A
Rabbit anti‐pRPA2 S4/S8	Bethyl	A300‐245A
Rabbit anti‐KAP1	Abcam	ab10484
Rabbit anti‐pKAP1 (Ser824)	Bethyl	A300‐767A
Rabbit anti‐SCAI antibody EPR4128	Abcam	124688
Rabbit anti‐Rad51 antibody EPR4030	Abcam	ab133534
Alexa Fluor 555 streptavidin	Thermo Fisher Scientific	S32355
rabbit anti‐streptavidin biotin conjugated	Rockland	200‐406‐095
Alexa Fluor 488 streptavidin	Thermo Fisher Scientific	S32354
rat anti‐BrdU	Abcam	ab6326
goat anti‐mouse Cy5.5	Abcam	ab6947
anti‐rat Alexa Fluor 555	Thermo Fisher Scientific	A21434
anti‐ssDNA	DSHB	AB10805144
anti‐goat Cy5.5	Abcam	ab6951