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. 2021 Sep 15;40(21):e107711. doi: 10.15252/embj.2021107711

Figure 3. 1,6‐hexanediol treatments (1,6HD) differentiate early and late viral replication factories.

Figure 3

  1. Replication factories in MA104‐NSP5‐EGFP cells infected with RV at 4 HPI dissolve after > 30 s post‐application of 4% (v/v) 1,6HD added to the cell culture medium (middle panel). Removal of 1,6HD results reassembly of multiple EGFP‐NSP5‐containing droplets dispersed in the cytosol (right panel). Bottom, replication factories at 12 HPI: before application of 1,6HD (left), 2 min after application (middle) and 15 min after removal of 1,6HD from cell culture medium (left). Note larger viral factories that remain refractory to 1,6HD treatment. Scale bar, 50 µm.
  2. Sensitivity of RV replication factories to aliphatic alcohols at 4 HPI. Left to right—1,6‐hexanediol (1,6HD); 1,3‐propylene diol (1,3PD); 1,2‐propylene diol, or propylene glycol (PG); glycerol (Gly); polyethylene glycol 200 (PEG200). Top panels— before application and bottom panels—1 min after application of these compounds (4.5% v/v). Scale bar, 30 µm.