TABLE 3.
Enzymes included in the model of the hydrogenosome metabolism
| Enzymea | Gene (protein ID)b | Mean expression (TPM)c | Localizationd | No. of other gut fungi where this gene was found |
|---|---|---|---|---|
| PFL 1 | 981064 | 1967 | Cytoplasm | 5 |
| PFL 2 | 1027775 | 182 | Cytoplasm | 5 |
| PFO | 623223 | 17 | Mitochondrion | 4e |
| Ac:SucCoA trans | 1731457 | 217 | Cytoplasm | 5 |
| Ac:SucCoA trans | 1316948 | 217 | Cytoplasm | 5 |
| SucCoA syn sub A | 1636158 | 1048 | Mitochondrion | 5 |
| SucCoA syn sub B | 1276456 | 1544 | Mitochondrion | 5 |
| Hydrogenase 1 | 1341048 | 219 | Mitochondrion | 5 |
| Hydrogenase 2 | 1718044 | 17 | Cytoplasm | 5 |
| Complex 1: nuoF | 1047445 | 339 | Mitochondrion | 5 |
| Complex 1: nuoE | 993995 | 519 | Mitochondrion | 5 |
| Complex 2: sub A | 1702000 | 4 | Mitochondrion | 5 |
| Complex 2: sub B | 1688149 | 13 | Mitochondrion | 5 |
| Complex 2: sub C | 1286787 | 12 | Mitochondrion | 3 |
| Complex 2: sub D | 1677752 | 8 | Mitochondrion | 2 |
| Fumarase | 985684 | 4 | Cytoplasm | 5 |
| ATP syn: sub alpha | 1037070 | 1 | Mitochondrion | 5 |
| ATP syn: sub beta | 1706307 | 8 | Mitochondrion | 5 |
| ATP syn: sub delta | 1045818 | 26 | Mitochondrion | 5 |
| ATP syn: sub gamma | 1061751 | 3 | Mitochondrion | 5 |
a
PFL, pyruvate formate lyase; PFO, pyruvate ferredoxin oxidoreductase; Ac, acetate; SucCoA, succinyl coenzyme A; syn, synthase; trans, transferase; sub, subunit.
b
ID, identifier.
c
TPM, transcripts per million. Transcriptomic expression count data are derived from the M2 cellobiose expression data set and represent the means from triplicates for each enzyme.
d
Localization was predicted using DeepLoc (55). Mitochondrial localization probably implies hydrogenosomal localization due to their evolutionary relationship (7).
e
Not identified in the genome of N. californiae; however, a transcript with close homology to PFO was identified.