FIG 2.

PCA of intestinal community composition in N2 wild-type (n = 164; black points) and mutant hosts colonized from a uniform metacommunity of eight bacterial species from the C. elegans native microbiome. (A to D) Subplots of the large ordination shown in its entirety in Fig. S3B; all worms except the constitutive daf-2 dauer mutant were grown to adulthood at 25°C. (A) Mild (eat-14; n = 69) and severe (phm-2; n = 78) grinder mutants; (B) p38 MAPK pathway-defective (AU37; n = 115) and derepressed (vhp-1; n = 84) mutants, with a glp-4 (n = 36) control for the AU37 strain; (C) TGF-β defective mutant (dbl-1; n = 69) and overexpression construct (ctls40; n = 48); (D) DAF-2/IGF defective (daf-16; n = 100) and derepressed (daf-2; n = 98) mutants. (E) Separate ordination, based on data from worms grown to adulthood at 16°C. When all worm hosts are grown to adulthood at 16°C, the double mutant CF1449 is indistinguishable from the daf-16 single mutant. Growth to adulthood at 16°C alters the later acquisition of microbial communities when adult worms (N2 and daf-16 strains) are colonized at 25°C. All data represent the results of single-worm digests and plating after 4 days total colonization on the uniformly distributed synthetic eight-species bacterial consortium. Ellipses for center of mass of the data associated with each host strain were generated using coord.ellipse (R package FactoMineR) with a confidence of 0.9999.