Unique stem cell lines identifier	ULLi002-A-49
	ULLi002-A-51
Institution	University of Louisiana at Lafayette, LA USA
Contact information of the reported cell line distributor	Baojin Ding (Baojin.Ding@Louisiana.edu)
Type of cell lines	iPSC
Origin	Human
Additional origin info (applicable for human ESC or iPSC)	Age: 30 YR Sex: Male Ethnicity: Asian
Cell Source	Skin fibroblasts.
Method of reprogramming	N/A
Clonality	Clonal
Evidence of the reprogramming transgene loss (including genomic copy if applicable)	RT/q-PCR
Cell culture system used	Serum-free and feeder-free medium
Type of Genetic Modification	Induced mutation
Associated disease	DYT1 dystonia
Gene/locus	TOR1A c.907_909delGAG (p.Glu303del)/9q34.11
Multiline rationale	Isogenic clones carrying heterozygous or homozygous of the same gene mutation.
Method of modification/site-specific nuclease used	CRISPR/Cas9
Site-specific nuclease (SSN) delivery method	Electroporated with a 4D-Nucleofector (Lonza) using CA-137 program.
All genetic material introduced into the cells	gRNA vector MLM3636 (Addgene #43860) Cas9 vector p3s-Cas9HC (Addgene #43945)
Unique stem cell lines identifier	ULLi002-A-49 ULLi002-A-51
Analysis of the nuclease-targeted allele status	Sequencing of the targeted allele
Method of the off-target nuclease activity surveillance	Targeted PCR/sequencing
Name of transgene	N/A
Eukaryotic selective agent resistance (including inducible/gene expressing cell-specific)	N/A
Inducible/constitutive system details	N/A
Date archived/stock date	4/29/2021
Cell line repository/bank	https://hpscreg.eu/cell-line/ULLi002-A-49 https://hpscreg.eu/cell-line/ULLi002-A-51
Ethical/GMO work approvals	Genetic modification was performed at Genome Engineering and iPSC Center (GEiC) at Washington University in St. Louis.
Addgene/public access repository recombinant DNA sources’ disclaimers (if applicable)	N/A