Fig. 4. VEGF blockade inhibits the vasculogenic potential of VEGFR1^high cells in vitro.

(a,c) Line graphs depicting the number of sprouts per high power field generated by DPSC or SHED. (b,d) Bar graphs showing the number of capillary-like sprouts at the end of the experimental period (i.e. 10 days). VEGFR1^high and VEGFR1^low DPSC or SHED cells were cultured in wells pre-coated with growth factor reduced Matrigel and stimulated with vasculogenic differentiation medium in presence of 0 or 25 μg/ml bevacizumab (anti-VEGF antibody). Different low case letters indicate statistical significance at p<0.05. Number of capillary sprouts (average +/− s.d.) is representative of 12 random microscopic fields from triplicate wells per condition. (e) Representative photomicrographs of the capillary sprouts observed after 10 days under the experimental conditions described above (bar: 100 μm).