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. Author manuscript; available in PMC: 2021 Nov 2.
Published in final edited form as: Methods Enzymol. 2019 Dec 19;632:309–337. doi: 10.1016/bs.mie.2019.11.012

Fig. 3.

Fig. 3

Definition of populations by traditional manual gating strategy applied to the analysis of 20-parameters flow cytometry data of a subcutaneous mouse tumor. (A) Initial gating strategy starting with Gate 1 to select the events acquired with stable flow stream, Gate 2 to exclude cell debris, and Gate 3 to exclude doublets. Strategy 1 and Strategy 2 depict options to select live CD45 positive cells. (B) Example of manual gating strategy to define specific populations starting from Gate 4′ or 5.