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. 2021 Oct 29;77(Pt 11):427–434. doi: 10.1107/S2053230X21010748

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type Hampton Research VDX 24-well plate
Temperature (K) 293
Protein concentration (mM) 0.5
Buffer composition of protein solution 10 mM HEPES–NaOH pH 7.5, 300 mM NaCl, 1 mM TCEP
Composition of reservoir solution 0.07 M sodium citrate pH 5.1, 10% glycerol, 5.6% PEG 4000
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (µl) 200