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. 2021 Nov 2;20:141. doi: 10.1186/s12943-021-01439-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mapping of DLC1 and PLCD1 interacting regions. A Co-immunoprecipitation of endogenous PLCD1, DLC1 and Caveolin-1 proteins in H1703 cells. The indicated antibodies for immunoprecipitation (IP) and Western blot (WB) were used. Input represents a fraction of the H1703 cell lysate before immunoprecipitation. B Schematic representation of the domain organization of DLC1 (top) and DLC1 constructs cloned as GFP-fusion proteins for immunoprecipitation in C. C PLCD1 Immunoprecipitation (IP) in cell lysates from H358 cells overexpressing control (GFP) or GFP-fusion plasmids spanning several regions in DLC1 followed by Western blot (WB) using the indicated antibodies. The expression of all GFP-fusion plasmids in total lysates is shown in the bottom panel. Asterisks (*) indicate the expression of the corresponding GFP-fusion proteins. D MBP pull-down (PD) in 293 T cells expressing GST control or GST PLCD1 using MBP control or MBP-DLC1 START 848–1091 purified proteins (from E.Coli), followed by GST Western blot (WB). The amount of MBP-purified proteins is shown as stained with Ponceau S (bottom panel). Input represents a fraction of the 293 T cell lysates before pull-down. E Schematic representation of the domain organization of PLCD1 (top) and PLCD1 constructs cloned as GST-fusion proteins to be used in F and G. F Glutathione pull-down (PD) in 293 T cells transfected with GST-PLCD1 plasmids encompassing several PLCD1 domains and GFP or GFP DLC1, followed by GFP and GST western blot. The expression of all GFP-fusion plasmids in total lysates is shown (bottom). G Pull-down (PD) using MBP DLC1 START 848–1091(purified from E. coli) and GST or GST-PLCD1 domains, followed by GST western blot. The amount of MBP- purified protein is shown as stained with Ponceau S. Expression of GST fusion proteins was checked by GST western blot in total lysates (bottom)