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. 2021 Nov 2;20:151. doi: 10.1186/s12944-021-01576-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — AICAR reduces C14 mediated apoptosis. A A fluorescence microscope was used to explore the mitochondrial membrane potential and apoptosis (n = 6, Scale bar = 100 μm). B The statistical analysis represents levels of red and green fluorescence (n = 6). C The expressions of Bcl-2, Bax, and Cleaved-caspase 3 were analyzed by immunoblotting in H9c2 (n = 6). D Bcl-2, Bax, and Cleaved-caspase3 expression results are shown (n = 6). E The expressions of Bcl-2 were tested by Immunofluorescence staining (n = 6; Scale bar represents as 100 μm). F The statistical analysis represents the positive expression of Bcl-2 in H9c2. Data are represented as means ± SD, two-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **P < 0.01, and ***P < 0.001