Fig. 3.

Dose-dependent Effect of Remdesivir on Mitochondrial and Nuclear Gene Expression. Panels A and C–H) HepG2 cells were exposed in culture to increasing concentrations of remdesivir for 24 and 48 h. RNA was extracted and RT-qPCR performed to quantify gene transcripts. Values are mRNA copies normalized to 18 s ribosomal RNA. B) HepG2 cells were exposed to a sub-toxic concentration of 2′-C-methyladeonsoine (25 μM 2-C-MA) as a positive control for mitochondrial RNA polymerase inhibition. Values are normalized to 18 s RNA and expressed as a percent of control. The plots represent the mean ± S.D. for 3 independent replications and asterisks indicate a statistically significant difference compared to the 0 μM dose group (Dunnett's p < 0.05).