Fig 6.

Hamster lungs demonstrate increased apoptosis and ferroptosis with SARS-CoV-2 infection. Heatmaps show relative differences and plots depict concentrations of (A) oxidized cardiolipins in lung tissue at 7 dpi and oxidized PE species in (B) BAL and (C) cells at 7 dpi. Data points represent individual animals. (D) Representative images from hamster lung tissue demonstrate increased TUNEL staining and increased staining for TFRC protein by immunohistochemistry (IHC) at 4, 7 and 14 dpi. n = 3 hamsters and/or group. (E) Immunoreactive cleaved caspase 3 increased at 4 and 7 dpi, and immunoreactive TFRC increased at 7 dpi in lung tissue lysates from hamsters infected with SARS-CoV-2. Below, densitometric analysis of cleaved caspase 3 and TFRC signal vs time, normalized to β-actin. n = 3 hamsters and/or group. Data shown as mean ± SEM. *P < 0.05 as determined by ANOVA with post-hoc Sidak's multiple comparisons test.