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. 2021 Sep 11;151(11):3431–3441. doi: 10.1093/jn/nxab270

FIGURE 2.

FIGURE 2

Differential abundance of the top 5 most abundant phyla and top 8 most abundant genera in human milk microbiota according to HMO composition by secretor strata. (A) Phylum secretor strata, (B) genus secretor strata, (C) phylum non-secretor strata, (D) genus non-secretor strata. Secretor, n = 78; non-secretor, n = 29. *Sialic acid and *Fucose denote that these HMO-bound molecules were run in the Poisson regression model as nmol/mL; all other HMOs were run as mg/mL, as indicated in the y-axis label. Heatmaps represent the results from multivariable Poisson regression models (PROC GENMOD; SAS Institute), adjusted for microbial DNA extraction and PCR sequencing batches with the top 5 phyla and top 8 genera as the outcome variables; the colored boxes refer to the incidence rate ratio on a log scale, while asterisks refer to statistically significant results. *P ≤ 0.022 for phylum, P ≤ 0.018 for genus. To account for multiple comparisons, the Benjamini-Yekutieli cut-point approach was used. DFLac, difucosyllactose; DFLNH, difucosyllacto-N-hexaose; DFLNT, difucosyllacto-N-tetrose; DSLNH, disialyllacto-N-hexaose; DSLNT, disialyllacto-N-tetraose; FDSLNH, fucodisialyllacto-N-hexaose; FLNH, fucosyllacto-N-hexaose; HMO, human milk oligosaccharide; LNFP I/II/III, lacto-N-fucopentaose-I/II/III; LNH, lacto-N-hexaose; LNnT, lacto-N-neotetraose; LNT, lacto-N-tetrose; LSTb/c, sialyl-lacto-N-tetraose-b/c; 2′FL, 2′-fucosyllactose; 3FL, 3-fucosyllactose; 3′SL, 3′-sialyllactose; 6′SL, 6′-sialyllactose.