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. 2021 Aug 12;151(11):3313–3328. doi: 10.1093/jn/nxab251

FIGURE 2.

FIGURE 2

The noncanonical activity of PKM2 promotes MPC proliferation. (A) Cell number in cultures transfected with shControl or shPKM2 (n = 3). (B) Cell number in cultures transfected with shControl or shPKM2 and supplemental pyruvate (n = 4). (C) PK enzyme activity (n = 4), (D) cell number (n = 3), (E) MTT absorbance (n = 4), and (F) percentage of dead cells (n = 4) in vehicle control or shikonin-treated cultures. (G) Cell numbers in cultures treated with vehicle control or shikonin and pyruvate (n = 3). (H) PK enzyme activity (n = 4), (I) cell number (n = 3), (J) MTT absorbance (n = 4), and (K) percentage of dead cells (n = 5) in vehicle control or TEPP-46 treated cultures. Values are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (asterisks indicate significant effects). AU, arbitrary units; GM, growth medium; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; PK, pyruvate kinase; shControl, short hairpin control; shPKM2, short hairpin pyruvate kinase M2.