FIG. 1.

Summary of the phenotypes of wild-type Hoxa9, PIM mutant panel, and DNA-binding mutant. Hoxa9 PIM consists of the amino acid sequence ANWL. Amino acid sequences of PIM mutants are indicated. Tag Hoxa9 is an N-terminal EE-tagged Hoxa9 with amino acid sequence EEYMPEA. Hoxa9-N51S is a DNA-binding mutant. DNA binding studies were performed with EE-tagged versions of wild-type Hoxa9, PIM, and N51S mutants due to the greater abundance of protein production in coupled transcription-translation; + or − refers to positive or negative scoring in an assay. For assay details and quantitative comparison of wild-type versus mutant performance, refer to the text and the following figures. n/d, not determined; n/a, not applicable.