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. 2000 May;20(9):3274–3285. doi: 10.1128/mcb.20.9.3274-3285.2000

FIG. 2.

FIG. 2

Hoxa9-immortalized cells differentiate morphologically into neutrophils or macrophages in response to G-CSF, M-CSF, or ATRA. Myeloid progenitors immortalized by Hoxa9 (clones HF1 and HF2) were cultured in GM-CSF (A and E), G-CSF for 72 h (B and F), M-CSF for 120 h (C and G), or GM-CSF plus ATRA for 5 days (D and H) and stained with Wright-Giemsa stain. Cells immortalized by wild-type Hoxa9 (clone HF1) were cultured in GM-CSF (I) or in G-CSF for 72 h. (J) and stained for NADPH oxidase activity. Cells immortalized by PIM mutant Hoxa9-Atet were cultured in GM-CSF (K) or in G-CSF for 48 h (L) and stained for NADPH oxidase activity. Myeloid progenitors immortalized by PIM mutant Hoxa9-Atet were cultured in GM-CSF (M), G-CSF for 72 h (N), or M-CSF for 120 h (O) and stained with Wright-Giemsa stain.