TABLE 1.
Morphology and NADPH oxidase expression of myeloid progenitor cell lines immortalized by HoxA9 and HoxA9 mutant proteins grown in the presence of GM-CSF and assayed in the presence of GM-CSF or after a shift to G-CSF for 48 h
| HoxA9 form | Morphologya in designated lymphokine (%)
|
% NADPH oxidase positive in designated lymphokine
|
||||||
|---|---|---|---|---|---|---|---|---|
| GM-CSF
|
G-CSF, 48 h
|
|||||||
| Blasts | Neut | Mac | Blasts | Neut | Mono | GM-CSF | G-CSF, 48 h | |
| HoxA9 HF1 | 89 | 10 | 1 | 6 | 88 | 6 | 15 | 87 |
| HoxA9 HF1.1 | 76 | 22 | 2 | 0 | 99 | 0.6 | 7 | 91 |
| HoxA9 HF1.4 | 82 | 17 | 1 | 0.7 | 99 | 0.3 | 5 | 95 |
| Tag A9 HF1 | 75 | 22 | 3 | 4 | 91 | 5 | 11 | 89 |
| Tag A9 HF2 | 85 | 14 | 1 | 8 | 88 | 4 | 15 | 85 |
| HoxA9-Atet | 86 | 14 | 0 | 6 | 92 | 2 | 19 | 97 |
| HoxA9-Gtet | 93 | 7 | 0 | 9 | 90 | 1 | 14 | 93 |
| HoxA9-WF-1 | 99 | 1 | <1 | 93 | 2 | 5 | 1.4 | 32 |
| HoxA9-WF-2 | 88 | 0 | 12 | 0 | 0 | 100 | 0.8 | 8 |
| HoxA9-WF-5 | 92 | 0 | 8 | 50 | 0 | 50 | 1 | 30 |
| HoxA9-WF-6 | 75 | 0 | 25 | 0 | 0 | 100 | 8.4 | 41 |
a
Neut, neutrophil; Mac, macrophage; Mono, monocyte.