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. 2021 Feb 9;4:47–59. doi: 10.1016/j.crphys.2021.02.003

Fig. 1.

Fig. 1

Increased Crym mRNA and μ-crystallin protein levels in Crym tg vs. control mice. A. Crym expression in several skeletal muscles and other tissues was analyzed from 3 Crym tg and 3 control mice. All analyses were in technical triplicate, except for soleus and diaphragm. B. Western blot of μ-crystallin in Crym tg and control TA muscle extracts. Control mice had 40 μg of TA homogenate per lane, and Crym tg lanes had 10 ​μg of TA homogenates per lane. We were unable to detect μ-crystallin reliably in controls in these blots. C. μ-Crystallin was quantitated with Protein Simple's Wes instrument. Areas under the curve, generated from electropherograms of μ-crystallin expression, were normalized to aconitase-2 expression (n ​= ​3, in technical duplicate) in several muscles and other tissues. Multiple individual t-tests using the Benjamini, Krieger and Yekutieli FDR approach at Q ​= ​1% determined statistical significance (∗∗∗∗ ​= ​p ​< ​0.0001). The results show that Crym tg skeletal muscle express high levels of Crym mRNA and protein compared to controls and to other tissues analyzed.