Fig. 3.

NH₄⁺does not modulate voltage-sensitive Ca²⁺(I_Ca) and hyperpolarization-activated (I_HCN) channels. Whole-cell voltage-clamp experiments were made in CA1 neurons from WT and RTT animals as indicated. A – Sample I_Ca traces were measured for 300 ms-long voltage steps from −70 to −50 mV before and 30 s after application of 1 mM NH₄⁺. B – Sample I_HCN traces were obtained in the same cells with voltage steps from −70 to −110 mV. The histograms on the right present group summary. The Student's t test in all cases gave p > 0.1 that indicated not significant (n.s.) differences. I_Ca currents in WT were yet distinctly bigger (−111 ± 7 vs. −191 ± 12 pA) and I_HCN currents were significantly smaller in RTT (−121 ± 8 vs. −81 ± 6 pA). For both sets of data P < 0.1 (the Mann-Whitney-U-test).